Leptin Receptor (LEPR) Goat Polyclonal Antibody

CAT#: TA302673

Goat Polyclonal Antibody against Leptin Receptor

Product Images

TA302673 (1.75µg/ml) staining of Human Cerebellum (A), and (1.5ug/ml) Mouse Foetal Brain (B) and Rat Brain (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

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  TA302673 (6µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

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  TA302673 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

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  TA302673 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo

Specifications

Product Data

• Applications: FC, IHC, PEP-ELISA, WB
• Recommended Dilution: ELISA: 1:16,000. WB: 1-3µg/ml.IHC:4µg/ml
• Reactivities: Human (Expected from sequence similarity: Dog)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-TQDDIEKHQSDAG, from the internal region of the protein sequence according to NP_002294.2; NP_001003679.1; NP_001003680.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 132.3 kDa
• Gene Name: leptin receptor
• Database Link:
  NP_002294
  Entrez Gene 450207 DogEntrez Gene 3953 Human
  UniProt ID P48357
• Background: Leptin (LEP; MIM 164160), an adipocyte-specific hormone that regulates adipose-tissue mass through hypothalamic effects on satiety and energy expenditure, acts through the leptin receptor (LEPR), a single-transmembrane-domain receptor of the cytokine receptor family. [supplied by OMIM]
• Synonyms: CD295; LEP-R; LEPRD; OB-R; OBR

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Neuroactive ligand-receptor interaction