Flotillin 1 (FLOT1) Goat Polyclonal Antibody

CAT#: TA302489

Goat Polyclonal Antibody against Flotillin 1

Product Images

TA302489 staining (0.3ug/ml) of H460 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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  HEK293 overexpressing FLOT1 (RC200231) and probed with TA302489 (mock transfection in first lane).

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  TA302489 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 0.3-1ug/ml; IHC: 3-5ug/ml; ELISA: 1:32,000
• Reactivities: Human (Expected from sequence similarity: Dog, Pig, Cow)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-SISQVNHKPLRTA, from the C Terminus of the protein sequence according to NP_005794.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50982 Da
• Gene Name: flotillin 1
• Database Link:
  NP_005794
  Entrez Gene 474831 DogEntrez Gene 10211 Human
  UniProt ID O75955
• Background: Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq]
• Synonyms: flotillin 1; integral membrane component of caveolae; OTTHUMP00000029208

Reference Data

• Protein Pathways: Insulin signaling pathway