BNIP3L Rabbit Polyclonal Antibody

CAT#: TA302077

Rabbit Polyclonal Antibody against BNIP3L

Product Images

The anti-NIP3 BH3 domain Pab (Cat. #TA302077) is used in Western blot to detect NIP3 BH3 in Ramos cell lysate (lane 1) and in mouse brain tissue lysate (lane 2).

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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  Freshly isolated mouse hepatocytes plated on coverslips (2 x105 cells/22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3). Fluorescence labeling of BNIP3 accomplished with anti-BNIP3 antibody TA302077. Data courtesy of Ruben Zamora, University of Pittsburgh.

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  IF image of HepG2 cells stained with BNIP3 (BH3 Domain Specific) antibody. HepG2 cells were incubated with TA302077 BNIP3 (BH3 Domain Specific) primary antibody (1:500, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: IF: 1:50~100, WB: 1:1000, IHC: 1:50~100
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This BNIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-187 amino acids from human BNIP3.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21541 Da
• Gene Name: BCL2/adenovirus E1B 19kDa interacting protein 3-like
• Database Link:
  NP_004322
  Entrez Gene 12177 MouseEntrez Gene 665 Human
  UniProt ID O60238
• Background: NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.
• Synonyms: BNIP3a; NIX

Reference Data

• Protein Families: Druggable Genome, Transmembrane