GFP Rabbit Polyclonal Antibody

SKU SP3005P

GFP rabbit polyclonal antibody, Purified

Product Data

• Application: IF, IP, WB
• Recommended Dilution: Immunoprecipitation: 10-20 µg/sample. ; Immunocytochemistry: 1-3 µg/ml. ; Western blot: 0.5-1.5 µg/ml. ; Positive Control: transfected cells. ; Negative Control: non-transfected cells.
• Reactivity: All Species
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: EGFP, a native full-length protein
• Specificity: The antibody reacts specifically with GFP, EGFP, EYFP fusion proteins in all species.
• Buffer: PBS; State: Purified; State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE); Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C. Do Not Freeze. ; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Database Link:
  P42212
• Background: Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (509 nm) when excited (excitation by Blue or UV light, absorption peak at 395 nm).; GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein.; GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.; To increase a fluorescence intensity of GFP, chromophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.
• Synonyms: Green fluorescent protein, GFP-Tag

Reference Data