GFP Rabbit Polyclonal Antibody
|Applications||IF, IP, WB|
|Recommended Dilution||Immunoprecipitation: 10-20 µg/sample.
Immunocytochemistry: 1-3 µg/ml.
Western blot: 0.5-1.5 µg/ml.
Positive Control: transfected cells.
Negative Control: non-transfected cells.
|Immunogen||EGFP, a native full-length protein|
|Specificity||The antibody reacts specifically with GFP, EGFP, EYFP fusion proteins in all species.|
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Preservative: 15 mM Sodium Azide
|Purification||Affinity Chromatography on Protein A|
|Storage||Store undiluted at 2-8°C. Do Not Freeze.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (509 nm) when excited (excitation by Blue or UV light, absorption peak at 395 nm).
GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein.
GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.
To increase a fluorescence intensity of GFP, chromophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.
|Synonyms||Green fluorescent protein, GFP-Tag|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.