RASSF1 (Isoform A) Mouse Monoclonal Antibody [Clone ID: 3F3]
Product Data | |
Clone Name | 3F3 |
---|---|
Application | ELISA, FC, IF, WB |
Recommended Dilution | The antibody has been tested by - Immunocytochemistry analysis (~ 1/100) - Flow Cytometry (2-5 µg for 1x106cells) - Western blotting (1/500~1/2,000) - ELISA to assure specificity and reactivity. |
Reactivity | Human |
Antibody Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Recombinant Human RASSF1A (1-340 aa), purified from E. coli. |
Specificity | The antibody recognizes Human RASSF1A. Other species not tested. |
Buffer | PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Shipping | Blue Ice |
Gene Name | Ras association domain family member 1 |
Database Link | |
Background | The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability. |
Synonyms | RASSF1A, RDA32, REH3P21, NORE2A |
Reference Data |
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