MAP2 Mouse Monoclonal Antibody [Clone ID: MT-08]

CAT#: SM3160P

MAP2 mouse monoclonal antibody, clone MT-08, Purified

Product Images

Western Blotting analysis (reducing conditions) of microtubules partially purified from porcine brain lysate. Lane 1: immunostaining with anti-MAP2ab (MT-01) Lane 2: immunostaining with anti-MAP2ab (MT-07) Lane 3: immunostaining with anti-MAP2ab (MT-08)

Specifications

Product Data

• Clone Name: MT-08
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: Immunohistochemistry on Frozen Sections (use mouse brain as positive tissue).
  Immunohistochemistry on Paraffin Sections:
  Recommended Dilution: 10 μg/ml.
  Positive tissue: brain.
  Immunocytochemistry (use Human neuroblastoma SH-SY5Y as positive control).
  Western blot (use Porcine brain or MAP2 as positive control).
  Immunoprecipitation (use Porcine brain as positive material).
  ELISA (use Porcine brain or MAP2 as positive control).
• Reactivities: Human, Porcine, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Microtubule protein (bovine brain) enriched for kinesin
• Specificity: The antibody recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab).
• Formulation: PBS with 15 mM Sodium Azide, approx. pH 7.4
  State: Purified
  State: Liquid purified Ig fraction (Purity > 95% by SDS-PAGE)
• Concentration: lot specific
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: microtubule associated protein 2
• Database Link:
  Entrez Gene 4133 Human
  UniProt ID P11137
• Background: MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.
• Synonyms: Microtubule-associated protein 2, MAP2, Neuronal Marker