SYK (5-360) Mouse Monoclonal Antibody [Clone ID: SYK-01]

SKU SM3127P

SYK (5-360) mouse monoclonal antibody, clone SYK-01, Purified

Product Data

• Clone Name: SYK-01
• Application: IF, IHC, IP, WB
• Recommended Dilution: Immunohistochemistry on Paraffin Sections (5 μg/ml).; Positive Tissue: Tonsil B cells.; Immunocytochemistry.; Immunoprecipitation.; Western blot (1-2 µg/ml, 60 min).; Positive Control: RBL rat basophilic leukemia cell line, A-431 human epidermoid carcinoma cell line, RAMOS lymphoma cell line, U-937 human histiocytic lymphoma cell line, JURKAT human peripheral blood T cell leukemia cell line.; Negative Control: HeLa human cervix carcinoma cell line.; Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate (1:1) with non-reducing SDS-PAGE sample buffer.; Application note: Non-reducing conditions. SDS-PAGE (12% separating gel).
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant fragment (aa 5-360) of Human Syk
• Specificity: The monoclonal SYK-01 antibody reacts with Protein Tyrosine Kinase p72Syk (Syk; Syk family tyrosine-specific phospho-transferase), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FceRI).
• Buffer: Phosphate buffered saline (PBS), pH~7.4; State: Purified; State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE); Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20 °C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: spleen tyrosine kinase
• Database Link:
  P43405
• Background: Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FceRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
• Synonyms: Tyrosine-protein kinase SYK, Spleen tyrosine kinase

Reference Data