LAT Mouse Monoclonal Antibody [Clone ID: LAT-01]

CAT#: SM3119P

LAT mouse monoclonal antibody, clone LAT-01, Purified

Product Images

Flow Cytometry analysis of LAT expression in peripheral blood lymphocytes using anti-human LAT (LAT-01) (surface markers were stained prior to intracellular staining of human LAT). Panel A: gate on CD4+ cells Panel B: gate on CD8+ cells

Specifications

Product Data

• Clone Name: LAT-01
• Applications: FC, IP, WB
• Recommended Dilution: Immunoprecipitation: Tyrosine-phosphorylated form of LAT is not optimally recognized.
  Flow cytometry: Recommended dilution: 1-4 µg/ml, intracellular staining. Tyrosine-phosphorylated form of LAT is not optimally recognized.
  Western blotting: Recommended dilution: 1-2 μg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT
• Specificity: The antibody reacts with Linker for activation of T cells (LAT), a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A affinity chromatography; purity: > 95 % (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: linker for activation of T-cells
• Database Link:
  Entrez Gene 27040 Human
  UniProt ID O43561
• Background: LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FceRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
• Synonyms: pp36, p36-38

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway