CD8A Mouse Monoclonal Antibody [Clone ID: MEM-31]
CD8A mouse monoclonal antibody, clone MEM-31, PerCP
|Recommended Dilution||Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension.
The content of a vial (1 ml) is sufficient for 100 tests.
The antibody does not react with formaldehyde-fixed cells; negative in Western Blotting application.
|Immunogen||Crude thymus membrane fraction|
|Specificity||The antibody MEM-31 recognizes a conformationally-dependent epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa).|
|Formulation||Phosphate buffered saline (PBS)
State: Liquid purified Ig fraction
Preservative: Phosphate buffered saline (PBS) solution containing 15mM sodium azide
Label: Conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
|Storage||Store undiluted at 2-8°C. DO NOT FREEZE! This products is photosensitive and should be protected from light.|
|Stability||Shelf life: one year from despatch.|
|Background||The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.|
|Synonyms||CD8 alpha chain, CD8A, MAL|