Neurofilament M (160 kD) Mouse Monoclonal Antibody [Clone ID: NF-09]
Neurofilament M (160 kD) mouse monoclonal antibody, clone NF-09, Purified
|Applications||IF, IHC, WB|
|Recommended Dilution||Western blot.
Immunohistochemistry on Paraffin Sections.
Positive Tissue: Neuro2A murine cell line.
Note: Carnoys fixative 2 x 3 min, blocking 1% Glycine + 0.2% Gelatin 10 min.
|Immunogen||Pellet of Porcine brain cold stable proteins after depolymerization of microtubules|
|Specificity||The antibody reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa) of various species.
Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
|Formulation||Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative.
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
|Purification||Affinity Chromatography on Protein A|
|Storage||Store undiluted at 2-8°C.
DO NOT FREEZE!
|Stability||Shelf life: one year from despatch.|
|Background||Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons, the longest cell protrusions known in vertebrates. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights (NF-H - heavy chain, NF-M - medium chain and NF-L - light chain). The three NF subunits share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm.|
|Synonyms||Neurofilament medium polypeptide, NF-M, NEF3, NEFM, Neurofilament 3, (Neuronal Marker)|
|The use of this Antibodies has been cited in the following citations:|
Microglial A20 Protects the Brain from CD8 T-Cell-Mediated Immunopathology
,Mohebiany, AN;Ramphal, NS;Karram, K;Di Liberto, G;Novkovic, T;Klein, M;Marini, F;Kreutzfeldt, M;Härtner, F;Lacher, SM;Bopp, T;Mittmann, T;Merkler, D;Waisman, A;,
Cell Rep 2020
,PubMed ID 32023471
Monoclonal antibody NF-09 specific for neurofilament protein NF-M
,Dráberová E, Sulimenko V, Kukharskyy V, Dráber P,
Folia Biol (Praha). 1999
,PubMed ID 10732731