Neurofilament M (160 kD) Mouse Monoclonal Antibody [Clone ID: NF-09]

CAT#: SM3068P

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Neurofilament M (160 kD) mouse monoclonal antibody, clone NF-09, Purified

USD 250.00

2 Weeks

    • 100 ug

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Product Data
Clone Name NF-09
Applications IF, IHC, WB
Recommended Dilution Western blot.
Immunohistochemistry on Paraffin Sections.
Positive Tissue: Neuro2A murine cell line.
Note: Carnoys fixative 2 x 3 min, blocking 1% Glycine + 0.2% Gelatin 10 min.
Reactivity All Species
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Pellet of Porcine brain cold stable proteins after depolymerization of microtubules
Specificity The antibody reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa) of various species.
Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Formulation Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative.
State: Purified
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration 1.0 mg/ml
Purification Affinity Chromatography on Protein A
Storage Store undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Background Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons, the longest cell protrusions known in vertebrates. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights (NF-H - heavy chain, NF-M - medium chain and NF-L - light chain). The three NF subunits share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm.
Synonyms Neurofilament medium polypeptide, NF-M, NEF3, NEFM, Neurofilament 3, (Neuronal Marker)
Reference Data

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