CD9 Mouse Monoclonal Antibody [Clone ID: MEM-61]
Product Data | |
Clone Name | MEM-61 |
---|---|
Application | FC, FN, IHC, WB |
Recommended Dilution | Flow Cytometry: 5 µg/ml Western blot (Non Reducing Conditions): 2-4 µg/ml. Functional application: The antibody induces FcgR-dependent platelet activation (aggregation). Immunohistochemistry on Paraffin Sections: 20 μg/ml. Positive tissue: prostate. |
Reactivity | Human |
Antibody Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Pre-B cell line NALM-6 |
Specificity | The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. |
Buffer | Phosphate Buffered Saline (PBS), approx. pH 7.4 without preservatives State: Azide Free State: Liquid (0.2 μm sterile filtered) purified Ig fraction (> 95% by SDS-PAGE) |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein A |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C. DO NOT FREEZE! |
Stability | Shelf life: one year from despatch. |
Shipping | Blue Ice |
Gene Name | CD9 molecule |
Database Link | |
Background | CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors. |
Synonyms | MIC3, TSPAN29, GIG2, p24, Tetraspanin-29, 5H9 antigen |
Reference Data |
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