CD9 Mouse Monoclonal Antibody [Clone ID: MEM-61]

CAT#: SM3039AS

CD9 mouse monoclonal antibody, clone MEM-61, Azide Free

Product Images

Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD9 (MEM-61) FITC. Total viable cells were used for analysis.

Specifications

Product Data

• Clone Name: MEM-61
• Applications: FC, FN, IHC, WB
• Recommended Dilution: Flow Cytometry: 5 µg/ml
  Western blot (Non Reducing Conditions): 2-4 µg/ml.
  Functional application: The antibody induces FcgR-dependent platelet activation (aggregation).
  Immunohistochemistry on Paraffin Sections: 20 μg/ml.
  Positive tissue: prostate.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Pre-B cell line NALM-6
• Specificity: The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
• Formulation: Phosphate Buffered Saline (PBS), approx. pH 7.4 without preservatives
  State: Azide Free
  State: Liquid (0.2 μm sterile filtered) purified Ig fraction (> 95% by SDS-PAGE)
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: CD9 molecule
• Database Link:
  Entrez Gene 928 Human
  UniProt ID P21926
• Background: CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
• Synonyms: MIC3, TSPAN29, GIG2, p24, Tetraspanin-29, 5H9 antigen