Bromodeoxyuridine / BrDU Mouse Monoclonal Antibody [Clone ID: MoBu-1]

SKU SM3005P

Bromodeoxyuridine / BrDU mouse monoclonal antibody, clone MoBu-1, Purified

Product Data

• Clone Name: MoBu-1
• Application: FC, IF, IHC
• Recommended Dilution: Flow Cytometry: 1-2 µg/ml.; Immunocytochemistry: 2 µg/ml.; Immunohistochemistry on Frozen and Paraffin Sections.
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: 5-bromodeoxyuridine conjugated with hemocyanine
• Specificity: The antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. ; The antibody MoBu-1 is also useful for detecting proliferating cells by Flow Cytometry or Immunofluorescence staining. It reacts also specifically with 5-Bromouridine (BrU).
• Buffer: PBS, pH 7.4, with 15 mM Sodium Azide as preservative; State: Purified; State: Liquid purified Ig fraction (> 95% pure by PAGE)
• Concentration: lot specific
• Purification: Protein A Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. ; Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Background: Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.

Reference Data