Anpep Rat Monoclonal Antibody [Clone ID: ER-BMDM1]

CAT#: SM2298P

Anpep rat monoclonal antibody, clone ER-BMDM1, Purified

Product Images

Staining of Mouse Peripheral blood Granulocytes with Rat anti-Mouse CD13 antibody

Specifications

Product Data

• Clone Name: ER-BMDM1
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/10-1/50 diluted CD13 antibody to label 10⁶ cells in 100 µl.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Balb/c bone marrow derived Macrophages.
  Cells from immunised rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.
• Specificity: This antibody Clone ER-BMDM1 Cat.-No SM2298P recognizes the Murine homologue of Human CD13, a 160kD cell surface antigen with Aminopeptidase N activity.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: alanyl (membrane) aminopeptidase
• Database Link:
  Entrez Gene 16790 Mouse
  UniProt ID P97449
• Background: In steady state mice, the CD13 antigen is expressed primarily by dendritic cell and mature macrophage subpopulations. The level of CD13 antigen expression increases with maturation, after the monocytic stage. Bone marrow cells and peripheral blood monocytes are reported to be CD13 negative while thioglycollate-elicited macrophages express high levels of CD13.
  Dendritic cells, interdigitating dendritic cells, veiled cells and Langerhans cells all express CD13, which is reported to be involved in the development of immature dendritic cells from monocytes.
• Synonyms: Aminopeptidase N, ANPEP, APN, PEPN