TCP1 eta (CCT7) Rat Monoclonal Antibody [Clone ID: PK/16/81a]

CAT#: SM2019P

TCP1 eta (CCT7) rat monoclonal antibody, clone PK/16/81a, Aff - Purified

Product Images

Western blot analysis of HeLa whole cell lysate (lane 1) and HeLa heat stressed whole cell lysate (lane 2) probed with rat anti CCT Eta Cat.-No. SM2019P followed by F (ab')2 rabbit anti rat IgG:HRP (Cat.No. SP1021HRP).

Specifications

Product Data

• Clone Name: PK/16/81a
• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500. Detects a band of approximately 60kD in Hela cell lysates.
  Immunohistochemistry on Paraffin Sections: 1/100.
  Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
  Recommended Positive Control: Human Prostate.
• Reactivities: Bovine, Human, Mouse, Rabbit
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody recognises the eta polypeptide of the CCT chaperonin molecule complex.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: chaperonin containing TCP1 subunit 7
• Database Link:
  Entrez Gene 10574 Human
  UniProt ID Q99832
• Background: The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.
• Synonyms: CCTH, CCT eta, NIP7-1