HLA Class I ABC Xenograft marker Rat Monoclonal Antibody [Clone ID: YTH862.2]

CAT#: SM2012P

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HLA Class I ABC Xenograft marker rat monoclonal antibody, clone YTH862.2, Purified



USD 556.00


Availability*
2 Weeks

Size
    • 200 ug


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Specifications

Product Data
Clone Name YTH862.2
Applications FC, FN, IF, IHC, IP
Recommended Dilution Immunoprecipitation.
Immunofluorescence.

Functional Assays: Removal of Sodium Azide by dialysis is recommended prior to use. 
Immunohistochemistry on Frozen Sections.
Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl.
Reactivities Human
Host Rat
Isotype IgG2b
Clonality Monoclonal
Immunogen PHA activated Human peripheral blood lymphocytes. 
Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.
Specificity This antibody recognizes a monomorphic determinant on the Human HLA Class I heavy chain.
Epitope analysis has shown that this antibody binds in the alpha-1 domain.
Antibody SM2012P detects the Human HLA class I ABC antigen without cross-reactivity to Mouse.
Studies have shown that this antibody may be able to induce apoptosis in activated but not resting T cells (See Genestier, L. et al. for details). 
Negative Species: Mouse.
Formulation PBS
State: Purified
State: Liquid purified IgG fraction from Tissue Culture Supernatant
Preservative: 0.09% Sodium Azide
Concentration lot specific
Purification Affinity Chromatography on Protein G
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.
Synonyms MHC class I ABC, HLA Class 2 ABC
Reference Data
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