HLA Class I ABC Xenograft marker Rat Monoclonal Antibody [Clone ID: YTH862.2]

CAT#: SM2012P

HLA Class I ABC Xenograft marker rat monoclonal antibody, clone YTH862.2, Purified

Product Images

1. Murine 4T1 cells were were mixed with Human DU-145 cells and stained with 1 g HLA Class I ABC antibody (Cat.-No SM2012P). Anti-Rat Cy3 was used as a secondary antibody. Red stained cells mark the HLA Class I ABC positive Human DU-145 cells. 2. Murine 4T

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  Flow Cytometry: Staining of Human peripheral blood lymphocytes with Rat Anti Human HLA ABC antibody (Cat.-No SM2012P)

Specifications

Product Data

• Clone Name: YTH862.2
• Applications: FC, FN, IF, IHC, IP
• Recommended Dilution: Immunoprecipitation.
  Immunofluorescence.
  Functional Assays: Removal of Sodium Azide by dialysis is recommended prior to use.
  Immunohistochemistry on Frozen Sections.
  Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10⁶ cells in 100 µl.
• Reactivities: Human
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: PHA activated Human peripheral blood lymphocytes.
  Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.
• Specificity: This antibody recognizes a monomorphic determinant on the Human HLA Class I heavy chain.
  Epitope analysis has shown that this antibody binds in the alpha-1 domain.
  Antibody SM2012P detects the Human HLA class I ABC antigen without cross-reactivity to Mouse.
  Studies have shown that this antibody may be able to induce apoptosis in activated but not resting T cells (See Genestier, L. et al. for details).
  Negative Species: Mouse.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.
• Synonyms: MHC class I ABC, HLA Class 2 ABC