Ctla4 Mouse Monoclonal Antibody [Clone ID: WKH203]

CAT#: SM1848P

Ctla4 mouse monoclonal antibody, clone WKH203, Purified

Specifications

Product Data

• Clone Name: WKH203
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: Flow cytometry: 1:10 - 1:20; use 10 µl to label 10e6 cells in 100 µl; membrane permeabilisation is required for this application.
  ELISA.
  Western blot.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified rCTLA-4hIg fusion protein. Spleen cells from immunised Balb/c were fused with cells of the X63-Ag8-653 myeloma cell line.
• Specificity: This antibody recognises CD152, also known as cytotoxic-T-lymphocyte antigen-4 (CTLA-4).
• Formulation: PBS containing 0.09 % Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cytotoxic T-lymphocyte-associated protein 4
• Database Link:
  Entrez Gene 63835 Rat
  UniProt ID Q62859
• Background: CD152 is similar in structure to CD28 and also binds ligands CD80 and CD86. CD152 is expressed by activated T-lymphocytes. Studies suggest that CD152 is also expressed by regulatory T-lymphocytes.
• Synonyms: CTLA-4