6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: AD1.1.10]

CAT#: SM1693B

6xHistidine Epitope Tag (HHHHHH) mouse monoclonal antibody, clone AD1.1.10, Biotin

Product Images

Western blot of HIS tagged protein probed with mouse anti Histidine Tag - Biotin (SM1693B) and visualised with Streptavidin - HRP

Specifications

Product Data

• Clone Name: AD1.1.10
• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blot: 1/1000.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: PAX6 transcription factor linked to Histidine tag
• Specificity: This antibody recognizes proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing Histidine tags.
  The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested.
  In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
  This antibody is routinely tested in Western blotting on Histidine tagged recombinant proteins and reacts against all Histidine-tagged proteins so far tested.
• Formulation: PBS
  Label: Biotin
  State: Liquid purified IgG fraction from tissue culture supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Biotin
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.