Cd68 Rat Monoclonal Antibody [Clone ID: FA-11]

CAT#: SM1550R

Cd68 rat monoclonal antibody, clone FA-11, PE

Product Images

Staining of Mouse peritoneal macrophages with Rat anti Mouse CD68 antibody -RPE (SM1550R) following permeablisation with Leucoperm.

Specifications

Product Data

• Clone Name: FA-11
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10⁶ cells in 100 µl (Membrane permeabilisation is required) .
  Rat anti mouse CD68 antibody, clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical studies, using both frozen and paraffin-embedded tissues ( Masaki et al. 2003) and Devey et al. 2009).
  Rat anti mouse CD68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified Concanavalin A acceptor glycoprotein from P815 cell line.
• Specificity: The antibody recognizes CD68.
  SM1550R does detect surface Macrosialin at low levels in resident Mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
• Formulation: PBS
  Label: PE
  State: Lyophilized purified IgG fraction from tissue culture supernatant
  Stabilizer: 1% BSA, 5% Sucrose
  Preservative: 0.09% Sodium Azide
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1.0 ml distilled water.
• Purification: Affinity Chromatography on Protein G
• Conjugation: PE
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD68 antigen
• Database Link:
  Entrez Gene 12514 Mouse
  UniProt ID P31996
• Background: Macrosialin is a heavily glycosylated transmembrane protein of 87-115kD, which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.
• Synonyms: Gp110, Macrosialin, Macrophage marker