Cd68 Rat Monoclonal Antibody [Clone ID: FA-11]

CAT#: SM1550B

Cd68 rat monoclonal antibody, clone FA-11, Biotin

Product Images

Staining of mouse peritoneal macrophages cells with Rat Anti Mouse CD68 antibody -Biotin.

Specifications

Product Data

• Clone Name: FA-11
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of Neat-1/10 dilueted antibody to label 10⁶ cells in 100 µl.
  Membrane permeabilisation is required for this application. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified Concanavalin A acceptor glycoprotein from P815 cell line
• Specificity: This CD68 antibody clone FA-11 does detect surface Macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
• Formulation: PBS, pH 7.4
  Label: Biotin
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Biotin
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD68 antigen
• Database Link:
  Entrez Gene 12514 Mouse
  UniProt ID P31996
• Background: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
  Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.
• Synonyms: Gp110, Macrosialin, Macrophage marker