CD81 / TAPA1 Hamster Monoclonal Antibody [Clone ID: Eat2]
Product Data | |
Clone Name | Eat2 |
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Application | ELISA, FC, IHC, IP, WB |
Recommended Dilution | Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl). Immunoprecipitation. Immunohistochemistry on Frozen Sections. ELISA. Western Blot. Not suitable for Paraffin Embedded Sections. |
Reactivity | Mouse, Rat |
Antibody Host | Hamster |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | 38C13, murine B cell line |
Specificity | This antibody recognises CD81, a 26kD cell surface glycoprotein. |
Buffer | PBS, pH 7.4 with 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction. |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G. |
Conjugation | Unconjugated |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Shipping | Blue Ice |
Gene Name | CD81 antigen |
Database Link | |
Background | In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Eat 2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding. |
Synonyms | S5.7; TAPA-1; TAPA1; Tetraspanin-28; Tspan-28; TSPAN28 |
Reference Data |
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