CD81 / TAPA1 Hamster Monoclonal Antibody [Clone ID: Eat2]

CAT#: SM1526PS

CD81 / TAPA1 hamster monoclonal antibody, clone Eat2, Purified

Specifications

Product Data

• Clone Name: Eat2
• Applications: ELISA, FC, IHC, IP, WB
• Recommended Dilution: ELISA.
  Western Blot.
  Flow Cytometry: Use 10 µl of 1/10-1/50 diluted antibody to label 10e6 cells in 100 µl.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  Not suitable for Paraffin Embedded Sections.
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: 38C13, murine B cell line.
• Specificity: This antibody recognises CD81, a 26kD cell surface glycoprotein.
• Formulation: PBS buffer pH 7.4 with 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD81 antigen
• Database Link:
  Entrez Gene 12520 Mouse
  UniProt ID P35762
• Background: In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation.
  Eat 2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.
• Synonyms: S5.7; TAPA-1; TAPA1; Tetraspanin-28; Tspan-28; TSPAN28