HLA Class I ABC Mouse Monoclonal Antibody [Clone ID: W6/32]

SKU SM1222P

HLA Class I ABC mouse monoclonal antibody, clone W6/32, Purified

Product Data

• Clone Name: W6/32
• Application: ELISA, FC, IF, IHC, IP
• Recommended Dilution: ELISA.; Immunoprecipitation.; Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 1x10⁶ cells or 100 µl whole blood.; This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.; Immunohistochemistry on Frozen Sections: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Acetone fixation for frozen sections is recommended.; Positive Control: Tonsil Tissue.; Does not work on Paraffin Sections.
• Reactivity: Bovine, Feline, Human, Monkey, Primate
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified human tonsil lymphocyte membranes
• Specificity: Antibody SM1222P recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha-3 and alpha-2 domains (See Ishitani, A. et al. for details). ; MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. ; Negative Species: Goat, Dog, Guinea Pig, Rabbit, Mouse, Chicken, Amphibia.
• Buffer: PBS, pH 7.4; State: Purified; State: Liquid purified IgG fraction from Tissue Culture Supernatant; Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Background: The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.
• Synonyms: MHC class I ABC, HLA Class 2 ABC

Reference Data