HLA Class I ABC Mouse Monoclonal Antibody [Clone ID: W6/32]
Product Data | |
Clone Name | W6/32 |
---|---|
Application | FC |
Recommended Dilution | Flow Cytometry: Use 10 µl of neat antibody to label 106 cells in 100 µl. This antibody is routinely tested in Flow Cytometry on Human peripheral blood lymphocytes. |
Reactivity | Bovine, Feline, Human, Monkey, Primate |
Antibody Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Membrane of human tonsil cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI/I-Ag4.1 myeloma cell line. |
Specificity | This antibody recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. Does not react with Mouse, Chicken, Rabbit, Dog, Goat and Guinea Pig. |
Buffer | PBS, pH 7.4 Label: FITC State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | FITC |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. |
Stability | Shelf life: one year from despatch. |
Shipping | Blue Ice |
Synonyms | MHC class I ABC, HLA Class 2 ABC |
Reference Data |
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