HLA Class I ABC Mouse Monoclonal Antibody [Clone ID: W6/32]

CAT#: SM1222F

HLA Class I ABC mouse monoclonal antibody, clone W6/32, FITC

Product Images

Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN HLA ABC:FITC (SM1222F).

Specifications

Product Data

• Clone Name: W6/32
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10⁶ cells in 100 µl.
  This antibody is routinely tested in Flow Cytometry on Human peripheral blood lymphocytes.
• Reactivities: Bovine, Feline, Human, Monkey, Primate
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Membrane of human tonsil cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI/I-Ag4.1 myeloma cell line.
• Specificity: This antibody recognises an antigenic determinant shared among products of the HLA A, B and C loci.
  Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells.
  Does not react with Mouse, Chicken, Rabbit, Dog, Goat and Guinea Pig.
• Formulation: PBS, pH 7.4
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Synonyms: MHC class I ABC, HLA Class 2 ABC