CD66b (CEACAM8) Mouse Monoclonal Antibody [Clone ID: 80H3]

CAT#: SM1154P

CD66b (CEACAM8) mouse monoclonal antibody, clone 80H3, Purified

Specifications

Product Data

• Clone Name: 80H3
• Applications: FC, IHC
• Recommended Dilution: Western Blot (See Feuk-Lagerstedt et al).
  Functional Studies: Removal of Sodium Azide by dialysis is recommended prior to use.
  Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 5x10⁵ cells or 100 µl whole blood.
  Immunohistochemistry on Frozen Sections: 1/25-1/100.
  The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Please use acetone fixation.
  Recommended Positive Control: Bone marrow tissue.
  Does not work on Paraffin Sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human leucocytes from individuals with CML
• Specificity: SM1154 recognizes the CGM6 gene product of the carcinoembryonic gene family.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Ascites
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: carcinoembryonic antigen related cell adhesion molecule 8
• Database Link:
  Entrez Gene 1088 Human
  UniProt ID P31997
• Background: CD66b is a 95-100 kD, GPI anchored molecule. CD66b is expressed strongly by mature granulocytes and meta-myelocytes, but only weakly by bone marrow myelocytes. Peripheral blood monocytes and lymphocytes do not express significant amounts of this antigen. The antibody reacts with cells from CML in the chronic phase. The CD67 cluster was re-designated as CD66b at the Vth Leucocyte Typing Workshop in Boston, 1993.
• Synonyms: CD67, CGM6, NCA-95