CD53 Mouse Monoclonal Antibody [Clone ID: MEM-53]

SKU SM1137PS

CD53 mouse monoclonal antibody, clone MEM-53, Purified

Product Data

• Clone Name: MEM-53
• Application: FC, FN, IHC, IP, WB
• Recommended Dilution: Immunohistochemistry (frozen sections): It is suitable for discrimination of lymphomas from other tumors. ; Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Leukocytes of pacient suffering from a LGL-type leukemia
• Specificity: The antibody reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells.; The antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
• Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4; State: Purified; State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography; purity: > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.; DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: CD53 molecule
• Database Link:
  P19397
• Background: CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
• Synonyms: Cell surface glycoprotein CD53, Tetraspanin-25, MOX44, TSPAN25

Reference Data