CD13 (ANPEP) Mouse Monoclonal Antibody [Clone ID: WM15]
CD13 (ANPEP) mouse monoclonal antibody, clone WM15, Purified
|Applications||ELISA, FC, FN, IHC, IP|
Flow Cytometry: 5 µg/ml.
Immunohistochemistry on Frozen Sections.
Functional Application: The antibody clone WM15 inhibits infection of cells by Human coronavirus and inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates.
|Immunogen||Human AML cells|
|Specificity||The antibody recognises the Human CD13 cell surface glycoprotein, a 150 kD molecule expressed by granulocytes and monocytes, and by myeloid leukaemia cells.|
|Formulation||PBS, pH~7.4 containing 15 mM Sodium Azide as preservative
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
|Purification||Affinity Chromatography on Protein G|
|Storage||Store undiluted at 2-8°C.
DO NOT FREEZE!
|Stability||Shelf life: one year from despatch.|
|Gene Name||alanyl aminopeptidase, membrane|
|Background||CD13 is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.
CD13 functions as an aminopeptidase enzyme and is also a receptor for coronavirus.
|Synonyms||Aminopeptidase N, ANPEP, APN, PEPN|
|The use of this Antibodies has been cited in the following citations:|
Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia
,McCormack E, MujiD M, Osdal T, Bruserud C, Gjertsen BT,
,PubMed ID 23243270
CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated
,Favaloro EJ, Browning T, Facey D,
Exp. Hematol. 1993
,PubMed ID 7902291
Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells
,Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Berndt MC, Musgrove E,
,PubMed ID 3865147
Myeloid progenitor surface antigen identified by monoclonal antibody
,Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Musgrove E,
Br. J. Haematol. 1985
,PubMed ID 4052321