CD9 Mouse Monoclonal Antibody [Clone ID: MM2/57]

CAT#: SM1065P

CD9 mouse monoclonal antibody, clone MM2/57, Purified

Product Images

Staining of acetone fixed frozen Human tonsil with Mouse anti-CD9 Antibody

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  Staining of Human peripheral blood platelets with Mouse Anti Human CD9.

Specifications

Product Data

• Clone Name: MM2/57
• Applications: FC, IHC, WB
• Recommended Dilution: Western Blot.
  Flow Cytometry: Use 10 µl of 1/100-1/200 diluted antibody to label 10⁶ cells or 100 µl whole blood.
  Immunohistochemistry on Frozen Sections: 1/500-1/1000.
• Reactivities: Bovine, Canine, Equine, Feline, Human, Rabbit
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human platelet membrane. Spleen cells from BALB/c mice immunised were fused with cells of the mouse SP2/0 myeloma line.
• Specificity: This antibody clone MM2/57 recognizes a conserved epitope on CD9 present on wide range of mammalian species.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD9 molecule
• Database Link:
  Entrez Gene 928 Human
  UniProt ID P21926
• Background: CD9 is a member of the tetraspan gene family.
  CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve.
• Synonyms: MIC3, TSPAN29, GIG2, p24, Tetraspanin-29, 5H9 antigen