PARC (CUL9) Rabbit Polyclonal Antibody

SKU R1518

PARC (CUL9) rabbit polyclonal antibody, Serum

Product Data

• Application: ELISA, IP, WB
• Recommended Dilution: This antibody reacts with human and mouse PARC by Western blot (1:500-1:1,000) and Immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3, and others). To date co-immuno-precipitation using 35SIP has been negative. A 281.2 kDa band corresponding to human PARC is detected. Most cell lines expressing PARC can be used as a positive control.
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 2503-2517 of Human PARC (C-terminus) coupled to KLH.
• Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.; This product reacts with human and mouse PARC.; Cross reactivity with PARC from other sources is not known.
• Buffer: State: Serum; State: Liquid (sterile filtered) with 0.01% (w/v) Sodium Azide as preservative.
• Concentration: lot specific
• Purification: Delipidation and defibrination.
• Conjugation: Unconjugated
• Storage: Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.; Avoid cycles of freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: cullin 9
• Database Link:
  Q8IWT3
• Background: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. PARC is a cullin family member that functions as a cytoplasmic anchor protein in p53-associated protein complexes. PARC regulates the subcellular localization of p53 and subsequent function. PARC forms a complex with p53 in the cytoplasm of unstressed cells and interacts with UBCH7 and UBCH8. PARC shows a cytoplasmic localization and is ubiquitously expressed in all tissues with highest expression in testis brain and kidney.
• Synonyms: CUL-9, H7AP1, KIAA0708, PARC

Reference Data