ZNF148 Rabbit Polyclonal Antibody

SKU R1493

ZNF148 rabbit polyclonal antibody, Serum

Product Data

• Application: ELISA, WB
• Recommended Dilution: This polyclonal antibody reacts human ZBP-89 in a variety of immunological assays including western blot and ELISA. This antibody is likely functional in immunohistochemistry and immunoprecipitation.; Recommended dilutions; Immunoblotting: 1:5,000. A band at approximately 89 kDa corresponding to human ZBP-89 is detected. Human monocytes or macrophages or nuclear extracts from PMA treated U937 cells can be used as a positive control.; ELISA: 1:10,000 - 1:30,000.
• Reactivity: Human
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified full length ZBP-89 recombinant protein expressed in E.coli
• Specificity: This polyclonal antibody is specific for ZBP-89.
• Buffer: State: Serum; State: Liquid (sterile filtered) Ig fraction containing 0.09% (w/v) Sodium Azide
• Concentration: lot specific
• Purification: Delipidation and defibrination
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted (in aliquots) at -20°C.; Avoid cycles of freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: zinc finger protein 148
• Database Link:
  Q9UQR1
• Background: The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The fulllength protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53.
• Synonyms: ZBP89

Reference Data