Alpha 1 Acid Glycoprotein (ORM1) Rabbit Polyclonal Antibody
Alpha 1 Acid Glycoprotein (ORM1) rabbit polyclonal antibody, Biotin, Purified
|Recommended Dilution||Western blot: 1/2,000-1/10,000.
This antibody has been assayed against 1.0 µg of AGP1 in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
|Immunogen||Alpha-1-acid glycoprotein (AGP1) from human plasma|
|Specificity||This antibody detects human Alpha-1-acid glycoprotein (ORM1 / AGP1).
Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified AGP1 from human plasma.
|Formulation||0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
State: Lyophilized purified Ig faction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% Sodium azide
|Reconstitution Method||Restore with 0.1 ml of deionized water (or equivalent).|
|Concentration||1.0 mg/ml (by UV absorbance at 280 nm)|
|Purification||Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Gene Name||orosomucoid 1|
|Background||Alpha 1 Acid Glycoprotein (AGP) is an acute phase plasma protein synthesized by the liver. The protein is believed to regulate the interaction between blood cells and endothelial cells, and together with haptoglobin and C reactive protein, also regulates the extravasation of the cells during infection and inflammation. AGP is a major acute phase reactant, both in human and in rat. In human plasma, AGP (44 kDa) is found at levels of 0.5-1.4 mg/ml. Its concentration in blood plasma is elevated during acute inflammation, such as trauma, malignancies, myocardial infarction, rheumatoid arthritis, bacterial infections, after major surgery, in cases of chronic pain etc, and can be used for the diagnosis of inflammatory conditions.|
|Synonyms||Orosomucoid-1, OMD1, Alpha-1-acid glycoprotein 1|