Collagen I (COL1A1) Rabbit Polyclonal Antibody
Collagen I (COL1A1) rabbit polyclonal antibody, Purified
|Applications||ELISA, IHC, IP, WB|
|Recommended Dilution||Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.
Specific researchers have reported that this antibody is also functional by conventional SDS-PAGE Western blot. See References below for additional details.
Western blot: 1/1,000-1/10,000.
|Reactivities||Bovine, Human, Mammalian, Mouse, Rat|
|Immunogen||Collagen type I purified from Human and Bovine placenta.|
|Specificity||This product has been prepared by Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, Human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
This antibody reacts with most mammalian Type I Collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other Human serum proteins or non-collagen extracellular matrix proteins is negligible.
|Formulation||0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0
State: Liquid (sterile filtered) purified Ig fraction
Preservative: 0.01% (w/v) Sodium Azide
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
For extended storage, mix with an equal volume of glycerol.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Predicted Protein Size||150 kDa (Target)|
|Background||Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens for immunization from human and bovine placenta and cartilage have been extensively purified by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS PAGE and immunoblotting.|
|Synonyms||COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen|
|Note||Note: Collagen type I consists of alpha-1 (139 kDa) and alpha-2 chains (129kDa). Since collagen type I is a triple helix consisting of one alpha-2 chain and two alpha-1 chains, one can expect bands of the dimeric (~270 kDa) and the trimeric form (~400 kDa). Remember that those chains are cross-linked and can't be broken by typical sample denaturation for SDS-PAGE.|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.