Ki67 (MKI67) (C-term) Rabbit Monoclonal Antibody [Clone ID: SP6]

CAT#: DRM004

Ki67 (MKI67) (C-term) rabbit monoclonal antibody, clone SP6, Supernatant

USD 1,173.00

2 Weeks*

    • 1 ml

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Product Data
Clone Name SP6
Applications IHC
Recommended Dilution Immunohistochemistry on Paraffin Sections: Use Ki67 antibody at a 1/50-1/100 dilution in an ABC method for 30 minutes at RT.  
Pretreatment: EDTA Buffer pH8.0
Recommended Positive Control: Tonsil.
Also suitable for Western blot.
Reactivities Human, Rat, Mouse
Host Rabbit
Isotype IgG
Clonality Monoclonal
Immunogen A synthetic peptide from C-terminus of Human Ki-67.
Specificity Reacts with Ki-67.
Cellular Localization: Nuclear.
Formulation State: Supernatant
State: Liquid Tissue Culture Supernatant
Preservative: ≤ 0.09% Sodium Azide
Conjugation Unconjugated
Storage Store undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Background Ki-67 antigen is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki-67 gene contains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis re-vealed multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quies-cent cells. Immunoblot analysis showed expression of 320- and 359-kD proteins. Sequence analy-sis predicted that the short-lived 2,896- and 3,256-amino acid protein isoforms contain potential nuclear targeting signals, over 200 potential phosphorylation sites, 19 N-myristoylation sites, 3 amidation sites, and numerous PEST sites. Antisense oligonucleotides inhibited cellular prolifera-tion in a dose-dependent manner, suggesting that Ki-67 protein expression may be an absolute requirement for cell proliferation.
Synonyms Ki67 antigen, MKI67, Proliferation marker
Reference Data

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