PROX1 (C-term) Rabbit Polyclonal Antibody

CAT#: DP3516P

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PROX1 (C-term) rabbit polyclonal antibody, Aff - Purified



USD 319.00


Availability*
2 Weeks

Size
    • 50 ug


Specifications

Product Data
Applications ELISA, IF, IHC, WB
Recommended Dilution Immunofluorescence: Use 1/750-1/1000 dilution and 1 µg/ml on cells.
Western Bloting: Use 1-5 µg/ml.
Immunohistochemistry.
Reactivities Human, Mouse, Rat
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Recombinant Human C-terminal part of the Homeobox Transcription Factor Prox-1 (homeo domain and prospero domain). (Cat.-No DA3565)
Specificity This antibody will detect Prox-1 in the nucleus of lymphatic endothelial cells by Immunostaining.
This antibody cross reacts with Equine Prox-1 in Immunohistochemistry using Paraffin-Embedded Sections (See Figure).
Formulation 5mM PBS, pH 7.2 without preservatives
State: Aff - Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore with distilled sterile water to a concentration of 0.1-1.0 mg/ml
Purification Antigen-Affinity Chromatography
Conjugation Unconjugated
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lymphatic vessels from blood vessels. Prox-1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells.
Synonyms PROX1
Reference Data
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