Lyve1 Rabbit Polyclonal Antibody
Lyve1 rabbit polyclonal antibody, Aff - Purified
|Applications||ELISA, FC, IF, IHC, WB|
|Recommended Dilution||ELISA: 1-15 µg/ml.
Western blot: 2-5 µg/ml.
FACS analysis: 3-10 µg/ml.
Immunohistochemistry on Paraffin and Frozen Sections: 0.25-4 µg/ml.
|Immunogen||Highly pure (> 95%) recombinant Mouse soluble LYVE-1 (Ala24-Gly228) produced in insect cells (Cat.-No DA3524).|
|Specificity||Cross reactivity of anti-Mouse Lyve-1 with Rat tissue.
The anti-Mouse Lyve-1 polyclonal antibody Cat.-No DP3513P shows a strong cross reaction with Rat Lyve-1 protein.
|Formulation||PBS, pH 7.4, without preservatives or stabilizers
State: Aff - Purified
State: Lyophilized purified IgG fraction
|Reconstitution Method||Restore in distilled sterile Water to a concentration of 0.1-1.0 mg/ml.|
|Purification||Antigen Affinity Chromatography|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.|
|Synonyms||LYVE1, CRSBP-1, CRSBP1, HAR, XLKD1|