Neuroglobin (NGB) Chicken Polyclonal Antibody

CAT#: DP2017

Neuroglobin (NGB) chicken polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western Blot.
  Immunohistochemistry.
  ELISA (for determination of the titer): The titer of this antibody, measured by indirect ELISA, is >1:100,000 for antibody concentration 1 mg/ml, 25 ng of antigen are coated per well, and defined at a point of maximal decrease of the titration curve.
• Reactivities: Canine, Human, Rat
• Host: Chicken
• Clonality: Polyclonal
• Immunogen: Recombinant human neuroglobin.
  The Human Neuroglobin is created as a recombinant protein produced in E. coli. It is the 17 kDa protein containing 150 amino acid residues of the Human Neuroglobin and one extra AA, N-terminal methionin.
  The amino acid sequence of the recombinant human neuroglobin is 100% homologous with the amino acid sequence of the human neuroglobin.
• Specificity: This antibody recognizes Neuroglobin.
• Formulation: Azide Free, 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
  Preservative: None
• Reconstitution Method: Restore with 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
• Concentration: 1.0 mg/ml (after reconstitution)
• Purification: Immunoaffinity chromatography on a column with immobilized recombinant Human Neuroglobin
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: neuroglobin
• Database Link:
  Entrez Gene 58157 Human
  UniProt ID Q9NPG2
• Background: Neuroglobin, a 151 amino acid reside protein, mainly expressed in vertebrate brain and retina, is a recently identified member of the globin superfamily. Augmenting O2 supply, neuroglobin promotes survival of neurons upon hypoxic injury, potentially limiting brain damage. Moreover, neuroglobin may be a novel oxidative stress-responsive sensor for signal transduction in the brain. Neuroglobin expression is increased by neuronal hypoxia in vitro and focal cerebral ischemia in vivo, and neuronal survival after hypoxia is reduced by inhibiting neuroglobin expression with an antisense oligodeoxynucleotide and enhanced by neuroglobin overexpression.
• Synonyms: NGB
• Note: Quality Control Test
  Indirect ELISA - to determine titer of the antibody.
  SDS PAGE - to determine purity of the antibody.