TIE2 (TEK) Mouse Monoclonal Antibody [Clone ID: Cl.16]

SKU DM3511

TIE2 (TEK) mouse monoclonal antibody, clone Cl.16, Purified

Product Data

• Clone Name: Cl.16
• Application: ELISA, FC, IHC, WB
• Recommended Dilution: ELISA: Use at 1-15 µg/ml.; Western blotting: Use at 1-2 µg/ml.; Flow Cytometry and Cell Sorting (FACS): Use at 2-5 µg/ml together with the appropriate secondary reagents. ; Immunohistochemistry: Use at 1-5 μg/ml (Cryo).
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human soluble extracellular domain of TIE-2 protein
• Specificity: The monoclonal antibody will detect native Human TIE-2/tek in ELISA experiments and on the surface of different cell types.
• Buffer: PBS, pH 7.4, without preservatives; State: Purified; State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatoghraphy on Protein G
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Gene Name: TEK receptor tyrosine kinase
• Database Link:
  Q02763
• Background: TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.
• Synonyms: TIE2, TIE-2, Angiopoietin-1 receptor, p140 TEK

Reference Data