CDC25A Mouse Monoclonal Antibody [Clone ID: DCS-120+DCS-121]

CAT#: DM337

CDC25A mouse monoclonal antibody, clone DCS-120+DCS-121, Purified

Product Images

Figure 1. Formalin fixed paraffin embedded human tonsil stained with CDC25A antibody. DM337

Specifications

Product Data

• Clone Name: DCS-120+DCS-121
• Applications: IF, IHC, IP, WB
• Recommended Dilution: Immunflourescence.
  Immunoprecipitation.
  Western Blot.
  Immunohistochemistry on Paraffin Embedded Sections:
  Use a dilution of 1/50-1/100 in an ABC method (30 minutes at room temperature, requires high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining)
  Recommended Positive Control: Tonsil.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: BALB/C mice were injected with purified recombinant CDC25A.
• Specificity: This antibody reacts with a 65 kD protein known as CDC25A.
  Cellular Localization: Nuclear membrane.
• Formulation: State: Purified
  State: Liquid purified Ig fraction with Sodium Azide as preservative.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cell division cycle 25A
• Database Link:
  Entrez Gene 993 Human
  UniProt ID P30304
• Background: The activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S phase and G2 for CDC25B. CDC25C is mainly expressed in G2 phase.
• Synonyms: CDC-25A