CDC25A Mouse Monoclonal Antibody [Clone ID: DCS-120+DCS-121]
| Product Data | |
| Clone Name | DCS-120+DCS-121 |
|---|---|
| Application | IF, IHC, IP, WB |
| Recommended Dilution | Immunflourescence. Immunoprecipitation. Western Blot. Immunohistochemistry on Paraffin Embedded Sections: Use a dilution of 1/50-1/100 in an ABC method (30 minutes at room temperature, requires high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining) Recommended Positive Control: Tonsil. |
| Reactivity | Human, Rat |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | BALB/C mice were injected with purified recombinant CDC25A. |
| Specificity | This antibody reacts with a 65 kD protein known as CDC25A. Cellular Localization: Nuclear membrane. |
| Buffer | State: Purified State: Liquid purified Ig fraction with Sodium Azide as preservative. |
| Conjugation | Unconjugated |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | cell division cycle 25A |
| Database Link | |
| Background | The activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S phase and G2 for CDC25B. CDC25C is mainly expressed in G2 phase. |
| Synonyms | CDC-25A |
| Reference Data | |
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