GDF15 (N-term) Mouse Monoclonal Antibody [Clone ID: ME-6D10]

CAT#: DM1224

GDF15 (N-term) mouse monoclonal antibody, clone ME-6D10, Purified

Product Images

Figure 2. Crified ME-1C4 monoclonal antibody. Lane 1: Molecular Weight marker, Lane 2: purified ME-1C4 antibody. Proteins were separated by CGE (Capillary Gel Electrophoresis, Agilent 2100 Bioanalyzer). Internal Control bands (240 kDa / 7 kDa / 4.5 kDa).

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  Figure.1: FACS analysis of BOSC23 cells using ME-6D1. BOSC23 cells were transiently transfected with anexpression vector encoding either MIC-1 (red curve) or anirrelevant protein (Control transfectant: black curve). Binding of ME-6D1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with MIC-1 trans-fected cells.

Specifications

Product Data

• Clone Name: ME-6D10
• Applications: ELISA, FC, WB
• Recommended Dilution: Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
  Flow Cytometry: 1.2 µg/10⁶ cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Genetic immunisation with cDNA encoding amino acids 30-194 (N-term) of MIC-1 proprotein.
• Specificity: Recognizes Human Macrophage Inhibitory Cytokine-1 (MIC-1).
  Other species not tested.
• Formulation: Phosphate buffered saline, pH 7.2 without preservatives
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: growth differentiation factor 15
• Database Link:
  Entrez Gene 9518 Human
  UniProt ID Q99988
• Background: Macrophage inhibitory cytokine-1 (MIC-1) is a member of the transforming growth factor-ß superfamily that regulates a wide variety of physiological processes involved in tissue differentiation and maintenance (1). MIC-1 is synthesized as a 62-kDa intracellular protein, which, after cleavage by a furin like protease, is secreted as a 25-kDa disulphide-linked dimeric protein. Expression of MIC-1 results in inhibition of macrophage activation, regulated by the p53 pathway, in response to pro-inflammatory monokines (1,2). MIC-1 is involved in tumour pathogenesis and its measurement can be used as a clinical tool for the diagnosis of a wide range of cancers. It could be a useful marker for aggressive prostate cancer while MIC-1 is upregulated in advanced and more aggressive prostatic tumours (3).
• Synonyms: GDF-15, MIC1, PDF, PLAB, PTGFB, Growth/differentiation factor 15, Placental bone morphogenetic protein, Placental TGF-beta, Macrophage inhibitory cytokine 1, MIC-1, Prostate differentiation factor, NSAID-activated gene 1 protein, NAG-1, NSAID-regulated gene 1 protein, NRG-1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein