FPRL1 (FPR2) Mouse Monoclonal Antibody [Clone ID: GM-1D6]
FPRL1 (FPR2) mouse monoclonal antibody, clone GM-1D6, Purified
|Applications||ELISA, FC, IF, WB|
|Recommended Dilution||Flow Cytometry: 1.2 µg/106 cells.
The antibody is routinely tested by Flow Cytometry on BOSC cells transiently transfected with an FPRL1 expression vector.
Immunofluorescence: 1 µg/106 cells.
Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
|Immunogen||GM1D6 was generated by genetic immunisation of BALB/c mice with a proprietary GPCR-immunisation vector containing the FPRL1 cDNA.
Selection: based on recognition of the complete native protein expressed on transfected mammalian cells.
|Specificity||GM1D6 recognizes FPRL1 transiently expressed on the cell surface of transfected BOSC cells (Fig.1).|
|Formulation||PBS, pH 7.2
State: Liquid purified IgG fraction
|Purification||Affinity Chromatography on Protein G|
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||Human formyl peptide-receptor-like-1 (FPRL1) belongs to the large family of G-protein coupled receptors (GPCR). It is a seven transmembrane protein expressed on mononuclear phagocytes and microglial cells. FPRL1 is a member of the chemoattractant subfamily of G protein-coupled receptors and plays a key role in inflammation via chemotaxis and the regulation of mediator release from leukocytes. It interacts with formyl peptides to attract phagocytes to sites of infection and promote inflammatory reactions (1). FPRL1 also interacts with amyloid beta peptides and has been implicated in phagocyte attraction to sites of amyloid plaques in Alzheimer’s disease (2). Since FPRL1 is expressed in neutrophils and monocytes, and it was shown using another monoclonal antibody that chemokines can be potent and specific ligands, FPRL1 might have interesting functions in inflammatory pathways (3).|
|Synonyms||N-formyl peptide receptor 2, FMLP-R-I, FPRH1, RFP, LXA4R|