CEACAM1 Mouse Monoclonal Antibody [Clone ID: 8G5]
CEACAM1 mouse monoclonal antibody, clone 8G5, Purified
|Applications||ELISA, FC, IF, IHC|
|Recommended Dilution||Flow Cytometry: 1.2 μg/106 cells.
Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
Immunohistochemistry on Cryosections: 10 μg/ml.
|Immunogen||Genetic immunisation with cDNA encoding the extracellular region of Human CEACAM1-A2.
Based on recognition of the complete native protein expressed on transfected mammalian cells.
|Specificity||This antibody recognizes Human CEACAM1 (biliary glycoprotein I, BGP/CD66a).
This antibody detects complete native protein expressed on transfected cells.
|Formulation||PBS, pH 7.2
State: Liquid purified Ig fraction
|Purification||Protein G Chromatography|
Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
|Stability||Shelf life: one year from despatch.|
|Background||CEACAM1 (BGP/CD66a) is a transmembrane glycoprotein which belongs to the carcinoembryonic antigen (CEA) gene family (1,2). It is expressed on cells of epithelial and myeloid origin and mediates homophilic intercellular interactions that influence cellular growth, immune cell activation, and tissue morphogenesis. CEACAM1 is a putative tumour suppressor based on diminished expression in some aggressive types of cancer cells (3). The anti-tumour effect may be due to inhibition of tumour angiogenesis, possibly by increased secretion of anti-angiogenic molecules from the cells (4). Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A (A1, A2) and one B domain. While the N, A1 and B domains can also be found in other CEA-family members, the A2 domain of CEACAM1 differs from those found in other CEACAM.|
|Synonyms||BGP, BGP1, Biliary glycoprotein 1|