TGF beta 1 (TGFB1) Mouse Monoclonal Antibody [Clone ID: TB21]

CAT#: DM1047

Reviews ()
Write a review

TGF beta 1 (TGFB1) mouse monoclonal antibody, clone TB21, Azide Free



USD 594.00


Availability*
2 Weeks

Size
    • 500 ug


Product images

Specifications

Product Data
Clone Name TB21
Applications ELISA, FN, IHC, WB
Recommended Dilution ELISA: This antibody recognizes both Human platelet-derived and recombinant TGF-beta in ELISA.
Functional Assay: This antibody neutralizes TGF-beta activity in vitro, in an inhibition assay of CCL/64 cell growth and neutralizes the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of micro-injection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo.
Immunohistochemistry on FFPE and Frozen Sections: This antibody has been used to demonstrate TGF-beta in ovine ovarian tissue and Human breast carcinoma at a dilution of 1/1000. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue.
Western Blotting: 5-20 ng/ml will allow visualization of 100 ng/lane of TGF-beta. 
In another Western Blot experiment the antibody used at 5-20 ng/ml, detected TGF-β at 200 ng/lane.
Reactivity Human, Sheep
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen TGF-beta from Human platelets.
Specificity This antibody detects Transforming Growth Factor beta/TGF beta. 
Western blotting demonstrated that this antibody reacts with the dimeric (25 kDa) and monomeric (12.5 kDa.) forms of TGF-β under both non-reducing and reducing conditions respectively. This antibody recognizes both human platelet-derived and recombinant TGF-β in ELISA.
Formulation 0.01M PBS, pH 7.0 without preservatives
State: Azide Free
State: Lyophilized purified IgG fraction
Reconstitution Method Restore in 0.5 ml double distilled water.
Concentration 1.0 mg/ml (after reconstitution)
Purification Affinity Chromatography on Protein G
Conjugation Unconjugated
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background TGF beta 1 (Transforming Growth Factor Beta 1) is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non neoplastic tissues, release transforming growth factors; and essentially all cells possess a specific TGF beta 1 receptor. The multimodal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function.
The C-terminal 112 amino acids constitute the mature protein.
Synonyms TGFB, Transforming growth factor beta-1, TGF-beta-1
Reference Data
Other products for "TGFB1"
Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.