Carboxypeptidase M (CPM) Mouse Monoclonal Antibody [Clone ID: M27]

SKU DDX0520P-100

Carboxypeptidase M (CPM) mouse monoclonal antibody, clone M27

Product Data

• Clone Name: M27
• Application: FC, IHC, IP
• Recommended Dilution: DDX0520P-50 / DDX0520P-100 Purified: FACS surface, ImmunoHistoChemistry frozen sections, Immunoprecipitation.; ; Usage recommendation: ; *This monoclonal antibody may be used between 5-20 μg/ml.; *Optimal dilution should be determined by each laboratory for each application.; *Coupled antibody: to maintain RT before using.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: pre-ALP cells (human B-lineage acute lymphoblastic leukaemia cell line)
• Specificity: Human Carboxypeptidase M.
• Buffer: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8; State: Purified
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Unconjugated
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.; Purified antibodies: avoid repeated freeze/thaw cycles.; Coupled antibodies: glycerol protects from freezing.
• Shipping: Blue Ice
• Gene Name: carboxypeptidase M
• Database Link:
  P14384
• Background: Monoclonal antibody (MoAb) M27 was generated after immunization of mice with the human B-lineage acute lymphoblastic leukemia cell line Pre-ALP. Under reducing conditions, MoAb M27 precipitated a 60- kD surface-membrane molecule from Pre-ALP cells. Expression cloning of Pre-ALP cDNA showed that M27 recognizes carboxypeptidase M (CPM), a cell-surface, zinc-dependent protease known to cleave off basic C-terminal amino acids from peptide hormones. Using M27 antibody, CPM was detected only at discrete B lymphocyte developmental stages, namely on committed precursors and on germinal center cells. CPM was also expressed on mature T cells, mainly after activation. These results provide the first description of a carboxy-peptidase on lymphoid cells. In addition, CPM was found on granulocytes and monocytes but not on their progenitors. CPM was found to be expressed with CD10 and CD13 respectively on lymphoid and myeloid cells. (de Saint Vis B et al., Blood 1995,86,1098-105).
• Synonyms: CPM

Reference Data