RPE65 Mouse Monoclonal Antibody [Clone ID: OTI6C6]
CAT#: CF815247
Carrier-free (BSA/glycerol-free) RPE65 mouse monoclonal antibody,clone OTI6C6
Formulation: Standard
Other products for "RPE65"
Specifications
Product Data | |
Clone Name | OTI6C6 |
Applications | WB |
Recommended Dilution | WB 1:500-1:2000 |
Reactivities | Human, Mouse |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Human recombinant protein fragment of human RPE65 (NP_000320) produced in E.coli. |
Formulation | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
Conjugation | Unconjugated |
Storage | Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws. |
Stability | Stable for 12 months from date of receipt. |
Predicted Protein Size | 61.4 kDa |
Gene Name | retinoid isomerohydrolase RPE65 |
Database Link | |
Background | The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital. [provided by RefSeq, Oct 2017] |
Synonyms | BCO3; LCA2; mRPE65; p63; rd12; RP20; sRPE65 |
Reference Data | |
Protein Families | Druggable Genome |
Protein Pathways | Retinol metabolism |
Documents
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FAQs |
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Resources
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.