RPE65 Mouse Monoclonal Antibody [Clone ID: OTI6D6]

CAT#: CF815232

Carrier-free (BSA/glycerol-free) RPE65 mouse monoclonal antibody,clone OTI6D6

Product Images

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human RPE65 plasmid (RC210433, lane 2), mouse RPE65 plasmid (MR223598, lane 3) using anti-ADAR antibody TA815232 (1:5000). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Western blot analysis of extracts (50ug) from mouse eye lysate by using anti-RPE65 monoclonal antibody(TA815232, 1:500)

Specifications

Product Data

• Clone Name: OTI6D6
• Applications: WB
• Recommended Dilution: WB 1:500-1:5000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment of human RPE65 (NP_000320) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.4 kDa
• Gene Name: retinoid isomerohydrolase RPE65
• Database Link:
  NP_000320
  Entrez Gene 19892 MouseEntrez Gene 6121 Human
  UniProt ID Q16518
• Background: The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital. [provided by RefSeq, Oct 2017]
• Synonyms: BCO3; LCA2; mRPE65; p63; rd12; RP20; sRPE65

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Retinol metabolism