IGF2BP1 Mouse Monoclonal Antibody [Clone ID: OTI1F7]

CAT#: CF815058

Carrier-free (BSA/glycerol-free) IGF2BP1 mouse monoclonal antibody,clone OTI1F7

Product Images

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1), human IGF2BP1 plasmid (RC216226, lane 2), human IGF2BP2 plasmid (RC205673, lane 3), human IGF2BP3 plasmid (RC209597, lane 4), mouse IGF2BP1 plasmid (MR209055, lane 5) using anti-IGF2BP1 antibody TA815058(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Western blot analysis of extracts (50ug per lane) from 5 cell lines lysates by using anti-IGF2BP1 monoclonal antibody(TA815058, 1:500)

Specifications

Product Data

• Clone Name: OTI1F7
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment of human IGF2BP1 (NP_006537) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.3 kDa
• Gene Name: Homo sapiens insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1), transcript variant 1, mRNA.
• Database Link:
  NP_006537
  Entrez Gene 140486 MouseEntrez Gene 10642 Human
  UniProt ID Q9NZI8
• Background: This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
• Synonyms: CRD-BP; CRDBP; IMP-1; IMP1; VICKZ1; ZBP1