CD9 Mouse Monoclonal Antibody [Clone ID: OTI3D5]

CAT#: CF813476

Carrier-free (BSA/glycerol-free) CD9 mouse monoclonal antibody, clone OTI3D5

Product Images

Flow cytometric analysis of living Hela cells, using anti-CD9 antibody(TA813476, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

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  Flow cytometric analysis of living K562 cells, using anti-CD9 antibody(TA813476, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

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  Flow cytometric analysis of living RPMI-8226 cells, using anti-CD9 antibody(TA813476, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

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  Flow cytometric analysis of living HUVEC cells, using anti-CD9 antibody(TA813476, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

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  Flow cytometric analysis of living U87MG cells, using anti-CD9 antibody(TA813476, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

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Specifications

Product Data

• Clone Name: OTI3D5
• Applications: FC
• Recommended Dilution: FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD9 (NP_001760) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.4 kDa
• Gene Name: CD9 molecule
• Database Link:
  NP_001760
  Entrez Gene 12527 MouseEntrez Gene 24936 RatEntrez Gene 928 Human
  UniProt ID P21926
• Background: This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]
• Synonyms: BTCC-1; DRAP-27; MIC3; MRP-1; TSPAN-29; TSPAN29

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Hematopoietic cell lineage