EEA1 Mouse Monoclonal Antibody [Clone ID: OTI2F6]

SKU CF813372

Carrier-free (BSA/glycerol-free) EEA1 mouse monoclonal antibody, clone OTI2F6

Product Data

• Clone Name: OTI2F6
• Application: WB
• Recommended Dilution: WB 1:500-1000
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-400 of human EEA1 (NP_003557) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 162.3 kDa
• Gene Name: early endosome antigen 1
• Database Link:
  NP_003557
  Q15075
• Background: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. [UniProtKB/Swiss-Prot Function]
• Synonyms: MST105; MSTP105; ZFYVE2

Reference Data

• Protein Pathways: Endocytosis