HNRPH1 (HNRNPH1) Mouse Monoclonal Antibody [Clone ID: OTI2E8]

CAT#: CF810861

Carrier-free (BSA/glycerol-free) HNRNPH1 mouse monoclonal antibody,clone OTI2E8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNRNPH1 (RC201834, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNRNPH1 (1:2000). Positive lysates LY417247 (100ug) and LC417247 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 8 different cell lines by using anti-HNRNPH1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HNRNPH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810861) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HNRNPH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810861) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HNRNPH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810861) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HNRNPH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810861) (1:500)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-HNRNPH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810861) (1:500)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HNRNPH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810861) (1:500)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HNRNPH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810861) (1:500)

• Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-HNRNPH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA (1:500)

• Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-HNRNPH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA (1:500)

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Specifications

Product Data

• Clone Name: OTI2E8
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human HNRNPH1 (NP_005511) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49 kDa
• Gene Name: Homo sapiens heterogeneous nuclear ribonucleoprotein H1 (HNRNPH1), transcript variant 2, mRNA.
• Database Link:
  NP_005511
  Entrez Gene 59013 MouseEntrez Gene 3187 Human
  UniProt ID P31943
• Synonyms: hnRNPH; HNRPH; HNRPH1