ROR gamma (RORC) Mouse Monoclonal Antibody [Clone ID: OTI3C9]

CAT#: CF809572

Carrier-free (BSA/glycerol-free) RORC mouse monoclonal antibody,clone OTI3C9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RORC (Cat# RC206541, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RORC (Cat# TA809572)(1:2000). Positive lysates LY401568 (100ug) and LC401568 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3C9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-266 of human RORC (NP_005051) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RAR related orphan receptor C
• Database Link:
  NP_005051
  Entrez Gene 19885 MouseEntrez Gene 368158 RatEntrez Gene 6097 Human
  UniProt ID P51449
• Background: The protein encoded by this gene is a DNA-binding transcription factor and is a member of the NR1 subfamily of nuclear hormone receptors. The specific functions of this protein are not known; however, studies of a similar gene in mice have shown that this gene may be essential for lymphoid organogenesis and may play an important regulatory role in thymopoiesis. In addition, studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: NR1F3; RORG; RZR-GAMMA; RZRG; TOR

Reference Data

• Protein Families: Druggable Genome, Transcription Factors